Have you wondered how scientists identify regions of similarity in three or more biological sequences? As described in my previous article, Sequence alignment is a method of arranging sequences of DNA, RNA, or protein to identify regions of similarity. In my latest article on bioinformatics, I have discussed about pairwise sequence alignment. Make sure to check them out as well. Multiple sequence alignment is quite similar to pairwise sequence alignment, but it uses three or more sequences instead of only two sequences.